|This is a digitally-processed image of tumor-derived |
exosomes (in green) in mice lungs during pre-metastatic
niche formation. (Credit: Héctor Peinado)
Understanding why a tumour metastasises in specific organs and do not in others is one of the top goals of oncology, and also one of the oldest. 126 years ago, the British physician, Stephen Paget, formulated his 'seed and soil theory', which advocates that metastasis requires the dispersal of tumour cells, 'seeds', as well as a welcoming environment, 'fertile soil', in the recipient organ. However, since then "the progress made in deciphering the mechanisms that guide metastasis to specific organs has been insufficient," write the authors in the report published in Nature.
In recent years, researchers from CNIO and Memorial Sloan Kettering Cancer Center have developed a theory that supports Paget's 'seed and soil' theory. They have collected evidence that tumours release millions of vesicles carrying representative samples of their proteins and genetic content. These are called exosomes and, like 'messenger vessels' or 'scouts', they are in charge of ensuring that the recipient organs are prepared to host the tumour cells. Specifically, the exosomes trigger the necessary molecular response - inflammation, vascularization, etc. - in the recipient organ to welcome the tumour cells, so that when they arrive they can proliferate.
But the researchers wanted to go even further. They knew that of the millions of exosomes originating from the tumour, only a few will nest and, moreover, they will not do so in any random organ, but in some more than others. Why? Could it be possible that the exosomes, the tumour 'scouts', have molecular labels that in some way direct them to specific organs?
To investigate this hypothesis, the authors selected 20 tumour cell lines from around ten different tumours, in which it is known that some metastasise to specific target organs; the lungs, liver, brain or bones. They analysed the proteins in their exosomes, nearly a thousand proteins, searching for those that could fulfil the role of a zip code.
They focused on a family of proteins called integrins, because these are present on the membrane of the exosomes where, theoretically, the destination 'label' should be found. This proved to be a sound strategy. From among a thousand proteins, they found that there were indeed specific combinations of integrins associated with metastasis to the lungs, and with metastasis to the liver.
"Our results suggest that there is a sort of 'zip code' on the surface of the exosomes that makes them go to specific organs and accumulate where the metastasis is going to occur," the researchers said.
If a tumour is 'tricked', by changing the destination code, it will colonise the organ that is specified. This has been tested with tumour cells that normally would go to the bones and, following the intervention of the researchers, went to the lungs. These data support that the 'soil' is as important as the 'seed' in the metastatic process.
Additional proof of the importance of integrins in metastatic nesting is that, as the study shows, when specific integrins are blocked in tumours that metastasize to specific organs - for example breast cancer to lungs and pancreas cancer to the liver - metastasis is reduced in these organs.
The researchers have also discovered the molecular signals that intercede in the reaction of the recipient tissue when the exosomes arrive. Specifically, these signals involve an increase in genes of the S100 family, which is known for provoking inflammatory signals; inflammation is a process associated with cancer.
These results represent the identification of potential new pharmacological targets, the researchers said: "We have defined a new type of mechanism for metastasis to specific organs that involves integrins and S100 proteins, which could be used as new anti-metastatic targets."
"In the future, we envisage the development of molecules to block combinations of integrins specifically in tumour tissues," the researchers concluded.
Based on material originally posted by CNIO.