Scientists have devised a technique for visualising tissues that could aid diagnosis and treatment of diseases including cancer. The new approach is more sensitive than existing tools and could help physicians detect illnesses at a much earlier stage, and may also be able to see how well a patient is responding to a treatment by monitoring changes occurring in cells.
A team led by the University of Edinburgh's MRC Centre for Inflammation Research has developed probes that light up specific targets inside a cell. Scientists tested the probes using fungal cells, but the same technique could easily be used to look at cells from patient samples. Description of their approach was published in the journal Nature Communications.
The probes are made up of a short molecule called a peptide - which recognises the target that researchers are trying to detect - attached to a new type of fluorescent tag. Researchers can track this tag using microscopes to see where the target is being produced by the cell.
Because the new probes are more sensitive than existing probes, researchers will be able to quantify exactly how much of the target is being produced by each cell. This will enable them to detect tiny changes in the molecular make-up of tissues that could be the early warning sign of a disease. Tracking these changes over time could also offer clues about how well a patient is responding to treatment.
The researchers say that one day their approach could help to improve patient scans so that diseases could be picked up faster using clinical imaging, such as PET scans.